Time Course Timecourse1 Timecourse2 Timecourse3 Yorf 0 5 10 15 20 30 40 50 60 0

Calculate the transcript half lives for the yeast genes using the 60 minute time series data provided in the file ‘DecayTimecourse.txt’. There are three sets of time series data (one data set is shown below and the rest are provided as additional columns in the same file)

Since there are three replicates the final half life can be calculated as the average of the three calculated half lives for each transcript.

Identify the genes/transcripts with very high (top 10%) and very low (bottom 10%) half lives.

Use R programming language.

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